Physiology of NIM interactios

CHRONO-NEUROIMMUNOPHARMACOLGY, USP, São Paulo, SP

Principal Investigators: Pedro Augusto Carlos Magno Fernandes, Zulma da Silva Ferreira e Regina Pekelmann Markus

Interests: The crosstalk between Immune and Chronobiological Systems focusing on the relevance of the immune-pineal axis in neuroinflammatory/ degenerative diseases and cancer.

Subjects: human samples (healthy,  major depression, cancer);  experimental models (rats, hamsters and mice). 

Methodologies:

- Multiplex - ELISAs: cytokines; melatonin; cortisol; leptin; 6-sulfatoximelatonin, etc.

- FACS:  co-localization; phagocytosis; cell identification; nuclear translocation; NF-kB, GR, etc.

- PCR and qPCR: melatonin enzymes and receptors related genes, cytokines, transcritptional factors, etc.

- HPLC: melatonin, indolamines and catecholamines

- cell culture: primary and lineage cells

Contact: rpmarkus@usp.br

NEUROIMMUNOMODULATION RESEARCH GROUP. FMVZ/USP, São Paulo, SP

Principal Investigators: João Palermo-Neto, Wanderley Moreno Quinteiro Filho e Atilio Sersun Caleffi.

 

Interests: the impact of environmental stressors on gut integrity, neuroendocrine, immune and microbiota in birds challenged or not with infectious agents. 

 

Subjects: poultry;  microbiota, antimicrobial agents, probiotics, innate and acquired immune system, Th1 x Th2 balance, intestinal integrity, experimental models. 

 

Methodologies:

- Stress-related behavioral responses (animal); 

- HPLC brain amine determinations 

- Macro and microscopic histopathological examinations

- Immunohistochemistry

- ELISAs: cytokines and corticosterone;

- FACS: immunophenotyping, cytokines, cell signaling (MAPK and NF-kB);

- Immune cell activity (lymphocytes proliferation and macrophage and neutrophils oxidative burst and phagocytosis)

- PCR and qPCR: stress-related genes, cytokines and cell signaling. 

Contact: jpalermo@usp.br

NEURO-IMMUNE INTERACTIONS RESEARCH GROUP. UNIFESP, São Paulo, SP

Principal Investigators: Alexandre Basso.

 

Interests: The crosstalk between the Immune and the Nervous Systems focusing on the regulation of the innate and the adaptive immune response by the sympathetic nervous system.

 

Subjects: experimental models  (several transgenic mouse strains). 

 

Methodologies:

- Multiplex and ELISAs

- Flow cytometry and cell-sorting

- PCR and qPCR, western blotting

- cell culture: mainly primary cells

- Cre-Lox system

- Animal models of inflammatory diseases

Contact: asbasso@unifesp.br