Physiology of NIM interactios
CHRONO-NEUROIMMUNOPHARMACOLGY, USP, São Paulo, SP
Principal Investigators: Pedro Augusto Carlos Magno Fernandes, Zulma da Silva Ferreira e Regina Pekelmann Markus
Interests: The crosstalk between Immune and Chronobiological Systems focusing on the relevance of the immune-pineal axis in neuroinflammatory/ degenerative diseases and cancer.
Subjects: human samples (healthy, major depression, cancer); experimental models (rats, hamsters and mice).
- Multiplex - ELISAs: cytokines; melatonin; cortisol; leptin; 6-sulfatoximelatonin, etc.
- FACS: co-localization; phagocytosis; cell identification; nuclear translocation; NF-kB, GR, etc.
- PCR and qPCR: melatonin enzymes and receptors related genes, cytokines, transcritptional factors, etc.
- HPLC: melatonin, indolamines and catecholamines
- cell culture: primary and lineage cells
NEUROIMMUNOMODULATION RESEARCH GROUP. FMVZ/USP, São Paulo, SP
Principal Investigators: João Palermo-Neto, Wanderley Moreno Quinteiro Filho e Atilio Sersun Caleffi.
Interests: the impact of environmental stressors on gut integrity, neuroendocrine, immune and microbiota in birds challenged or not with infectious agents.
Subjects: poultry; microbiota, antimicrobial agents, probiotics, innate and acquired immune system, Th1 x Th2 balance, intestinal integrity, experimental models.
- Stress-related behavioral responses (animal);
- HPLC brain amine determinations
- Macro and microscopic histopathological examinations
- ELISAs: cytokines and corticosterone;
- FACS: immunophenotyping, cytokines, cell signaling (MAPK and NF-kB);
- Immune cell activity (lymphocytes proliferation and macrophage and neutrophils oxidative burst and phagocytosis)
- PCR and qPCR: stress-related genes, cytokines and cell signaling.
NEURO-IMMUNE INTERACTIONS RESEARCH GROUP. UNIFESP, São Paulo, SP
Principal Investigators: Alexandre Basso.
Interests: The crosstalk between the Immune and the Nervous Systems focusing on the regulation of the innate and the adaptive immune response by the sympathetic nervous system.
Subjects: experimental models (several transgenic mouse strains).
- Multiplex and ELISAs
- Flow cytometry and cell-sorting
- PCR and qPCR, western blotting
- cell culture: mainly primary cells
- Cre-Lox system
- Animal models of inflammatory diseases